Featuring reliable, up-to-the-minute reviews of major developments in chromatography, Volume 43 provides detailed discussions of current topics, issues, and developments in separation science. The book discusses the utilization and application of new analytical methodologies. It covers subjects such as gradient elution in liquid column chromatography, solid phase microextraction, and polyelectrolytes. It also examines supercritical fluids for off-line sample preparation in food analysis and assess technologies for the analysis of biologically important compounds. Each chapter includes an abundance of bibliographic citations.